INTRODUCTION:

Man in solving its numerous medical challenges for ages depends on his immediate environment taking advantages of nature’s provisions of its beauty for life and survival. They learned to depend on plants and in some cases animals in providing solutions to the myriad of their health problems. Aside from these, there has been an increasing incidence of multiple resistances in human pathogenic microorganisms in recent years, largely in discriminate use of commercial antimicrobial drugs commonly employed in the treatment of infectious diseases[1]. This has forced scientist to search for new antimicrobial substances from various sources like medicinal plants [2] (Antessa 2008.Bougainvillea glabra is a common flower planted everywhere in the Philippines, it is used in landscaping and beautification of Filipino houses. Since the ancient times, it is not known for any medicinal uses in the country. The people are left clueless about the wonders of healing this flower could bring to them [3].

Preparation of standard stock solution:

Standard stock solution was prepared by dissolving accurately weighed 10 mg methanol extract in 10ml distil water and it gives 1000μg/ml drug concentration.1 ml was diluted to 10ml with water. It gives 100μg/ml drug solution. 1ml of 100μg/ml was diluted to 10 ml to produce the concentration of 10 μg/ml. The absorbance of resulting solution was measured against blank solution in the UV region of 200-400nm, which shows maximum absorbance at 378nm.

Preparation of stock solution:

Standard Preparation of stock solution:

Standard stock solution was prepared by dissolving accurately weighed 10mg of Bougainvillea glabra powder in 10 ml of water and the volume was made up to mark of volumetric flask. It gives 1000 μg/ml of drug solution. 1 ml of this stock solution was diluted to 10ml with water. It gives 100 μg/ml of drug solution. 1ml of 100 μg/ml was diluted to 10ml to produce the concentration of 10 μg/ml. the absorbance of resulting solution was measured against blank solution in the UV region of 200-400nm, which shows maximum absorbance at 368.5nm.

Preparation of calibration graph:

Different aliquots of standard solution of 100μg/ml were transferred in to series of 10 ml volumetric flask the volume in each flask were made up to 10m with water and absorbance were measured at 368.5nm against solvent blank. The obtained absorbencies when plotted against the concentration of methanol extract gives calibration graph.

Detection of amino acid by TLC method:

In TLC preparation initially design the measured size 10x2cm and draw the base line the prepared sample were put on the base line and placed in to the TLC chamber. After completion of saturation then drying the plate 40-60°C in hot air oven. After completion of drying then spraying the detecting reagents on TLC plates and again put in to the hot air oven for drying then then elution of spot shows pink colour then it is confirmed as amino acid by RF value 0.88 .in next step whatever TLC eluting spots measured the solute travelling length and solvent travelling length spots measured and obtained RF value is 0.7.

Theoretically Rf value lies in between 0-1

Rf = distance travelled by solute front/ distance travelled by solvent front= 3.5/5=0.7

Experimentally obtained Rf value= 0.7

RESULTS AND DISCUSSION:

The phytochemical constituents of Bougainvillea glabra extract shows presence of alkaloids, saponins, tannins, reducing sugar and cardiac glycosides in the flowers of Bougainvillea glabra. Antrhaquinones, flavonoids and terpenoids were absent in the flowers of Bougainvillea glabra. The values obtained at different stages were determined. The chromatography Rf value is 0.7.then the standard graph obtained by the sample is Y=0.oo3x+o.ooo1 the stock solution of drug sample identified by UV spectrum are λmax of Bougainvillea glabra is 368.5nm. [4-10]

CONCLUSION:

In industry pharmacognosy plays a major role in bringing out a safe and effective dosage form. The project work has given the importance for studying the phytochemical screening and analytical studies of Bougainvillea glabra. The phytochemical constituents of Bougainvillea glabra extract shows various chemical constituents present in aqueous extract are alkaloids, amino acids, carbohydrates, volatile oils, condensed tannins, steroids, terpenoids, glycosides are present, proteins, starch, condensed tannins, steroids. the present study was carried out to determine the phytochemical like alkaloids, tannins, saponins and amino acids are present in methanol extract of Bougainvillea glabra along with estimated the Rf Value amino acid and spectral study used to determine the λmax. The Rf Value of amino acid is 0.7, spectrum of alkaloid of λmax is 368.5nm, standard graph of water extract linearity curve is y=0.0003x+0.0001.

REFERENCES:

1 Agnieszka N, Jan DY, Norbert B (2008). Flavonoid content and antioxidant activity of caraway roots (Carum carvi L.). Veg. Crop Res. Bull., 68: 127-133.Antessa (2008). Phytochemical constituents, Antibacterial Activity Index and Phytocontrol Potentials of Three 124

2. Minty Herbal Plants. Unpublished Science Investigatory Project presented to 9thNational Science Quest at Baguio City, Philippines.

3 Asker VSABE, Berg VDDJ, Tromp MNJL (1996). Structural aspects of antioxidant activity of flavonoids. Free Radic. Biol. Med., 20: 331-42

4 Khandelwal KRS. Practical Pharmacognosy, 17th edition, 2007, 40-48.

5 Ashok K. Guptha, Introduction to Pharmaceutics-I, 3rd edition, pg.no.154-172.

6 Agrawal SS, M. Paridhavi, Herbal Drug Technology, 1st edition 2007,pg.no.450-459.

7 Kokate CK. Practical Pharmacognosy, 4th edition, 2006, 379-384.

8 Sharma YR. Elementary Organic Spectroscopy, 4th edition, 2011, 170-174.

9 Ravi Sankarv S. Text book of Pharmaceutical Analysis, 3^rd Edition, 2006, 46-49.

Received on 26.06.2013 Accepted on 24.07.2013

Asian J. Pharm. Ana. 3(3): July-Sept. 2013; Page 83-85